Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein
Enhanced green fluorescence protein (EGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). EGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP fluorescence is stable under fixation conditions and suitable for a variety of applications. EGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize EGFP-tagged proteins within living cells without the need for chemical staining. Other applications of EGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. EGFP technnology has considerably contributed to a greater understanding of cellular physiology. Description: EGFP produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27kDa. EGFP is purified by proprietary chromatographic techniques
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB: 1:1000~1:5000 IP: 1:100~1:200
Application Notes:
EGFP polyclonal Antibody detects endogenous levels of EGFP protein.
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