Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities. PI3K Class II alpha generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate. PI3K Class II alpha is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles. Research studies have indicated that PI3K Class II alpha regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network. PI3K Class II alpha also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin. PI3K Class II alpha has also been shown to regulate neurosecretory granule exocytosis and vascular smooth muscle contraction. Unlike other PI3K family members, PI3K Class II alpha is less sensitive to the PI3K inhibitors wortmannin and LY294002.
The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB 1:2000, IHC 1:150
Application Notes:
IgG1
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