TIM23 is a subunit of the mitochondrial import inner membrane translocase complex. This complex mediates translocation of presequence-containing proteins into the mitochondrial matrix and inner membrane. Besides TIM23, there are multiple subunits in the complex, including TIM17A, TIM17B, and TIM50 (1,2). Studies show that mutant huntingtin (mHTT), the protein causing neurodegenerative Huntingtons disease, binds to TIM23 subunit. There are less soluble matrix mitochondrial proteins imported by the TIM23 complex in cells expressing mHTT and in the brain tissues of Huntingtons disease patients when compared with controls (2). In addition, asbestos causes translocation of NOX4 to the mitochondrial matrix through direct interaction with TIM23 in lung macrophages. TIM23 increases NOX4-induced reactive oxygen species (ROS) in mitochondria and metabolic reprogramming to oxidative phosphorylation (3).
The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB 1:500~2000, IHC 1:2000
Application Notes:
IgG2b
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