ERK1/2 (Phospho-T202/Y204) Rabbit monoclonal antibody, Unconjugated

Product Overview

• Article Name: ERK1/2 (Phospho-T202/Y204) Rabbit monoclonal antibody, Unconjugated
• Biozol Catalog Number: BWT-MB66339
• Supplier Catalog Number: MB66339
• Alternative Catalog Number: BWT-MB66339-50UL,BWT-MB66339-100UL
• Manufacturer: Bioworld Technology
• Host: Rabbit
• Category: Antikörper
• Application: IP, WB
• Species Reactivity: Human, Rat
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human Erk1
• Conjugation: Unconjugated
• Alternative Names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2

Product Description

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Product Properties

• Molecular Weight: ~ 42, 44 kDa
• UniProt: P27361
• Purity: The antibody was purified by immunogen affinity chromatography.
• Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Application Notes

• Application Dilute: WB (1/500 - 1/1000), IP (1/10 - 1/50)
• Application Notes: Recognizes endogenous levels of ERK1/2 (pT202/Y204) protein.