ACLY monoclonal antibody, Unconjugated, Mouse

Product Overview

• Article Name: ACLY monoclonal antibody, Unconjugated, Mouse
• Biozol Catalog Number: BWT-MB66502
• Supplier Catalog Number: MB66502
• Alternative Catalog Number: BWT-MB66502-50UL,BWT-MB66502-100UL
• Manufacturer: Bioworld Technology
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, WB
• Species Reactivity: Human, Monkey, Mouse
• Immunogen: Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
• Conjugation: Unconjugated
• Alternative Names: ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme

Product Description

ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme .

Product Properties

• Molecular Weight: ~ 125 kDa
• UniProt: P53396
• Purity: The antibody was purified by immunogen affinity chromatography.
• Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application Notes

• Application Dilute: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)
• Application Notes: Recognizes endogenous levels of ACLY protein.