BrdU monoclonal antibody, Unconjugated, Mouse

Product Overview

• Article Name: BrdU monoclonal antibody, Unconjugated, Mouse
• Biozol Catalog Number: BWT-MB9001
• Supplier Catalog Number: MB9001
• Alternative Catalog Number: BWT-MB9001-50UL,BWT-MB9001-100UL
• Manufacturer: Bioworld Technology
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF
• Species Reactivity: All
• Immunogen: Recombinant full length Human BrdU.
• Conjugation: Unconjugated
• Alternative Names: BrdU, Bromodeoxyuridine

Product Description

The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a

Product Properties

• Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Application Notes

• Application Dilute: ELISA: 0.015~ 0.05 µg/ml Flow cytometry: 1~3 µg for 1x106 cells ICC/IF: 1~3 µg/ml
• Application Notes: The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2-deoxyuridine (BrdU), 5-chloro-2-deoxyuridine (CldU) and 5-iodo-2-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2-deoxyuridine (F