Recombinant Human SDF-1 alpha (rHuSDF-1 alpha/CXCL12- alpha)

Product Overview

• Article Name: Recombinant Human SDF-1 alpha (rHuSDF-1 alpha/CXCL12- alpha)
• Biozol Catalog Number: BWT-PR1112
• Supplier Catalog Number: PR1112
• Alternative Catalog Number: BWT-PR1112-2UG,BWT-PR1112-10UG,BWT-PR1112-1.0MG
• Manufacturer: Bioworld Technology
• Category: Proteine/Peptide

Product Description

SDF-1alpha and SDF-1beta, members of the chemokine alpha subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1alpha and SDF-1beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1alpha and SDF-1beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1beta and absent from SDF-1alpha. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils.

Product Properties

• Molecular Weight: 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
• Source: Escherichia coli.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
• Formula: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 130mM NaCl.

Application Notes

• Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.