Recombinant Human soluble CD40 Ligand (rHusCD40L) Preis auf Anfrage

Product Overview

• Article Name: Recombinant Human soluble CD40 Ligand (rHusCD40L) Preis auf Anfrage
• Biozol Catalog Number: BWT-PR1113
• Supplier Catalog Number: PR1113
• Alternative Catalog Number: BWT-PR1113-10UG,BWT-PR1113-50UG,BWT-PR1113-1.0MG
• Manufacturer: Bioworld Technology
• Category: Proteine/Peptide

Product Description

CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

Product Properties

• Molecular Weight: Approximately 16.3 kDa. a single non-glycosylated polypeptide chain containing 149 amino acids.
• Source: Escherichia coli.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
• Formula: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.0.

Application Notes

• Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.