Recombinant Human Thymic Stromal Lymphopoietin (rHuTSLP)

Product Overview

• Article Name: Recombinant Human Thymic Stromal Lymphopoietin (rHuTSLP)
• Biozol Catalog Number: BWT-PR1121
• Supplier Catalog Number: PR1121
• Alternative Catalog Number: BWT-PR1121-2UG,BWT-PR1121-10UG,BWT-PR1121-1.0MG
• Manufacturer: Bioworld Technology
• Category: Proteine/Peptide

Product Description

TSLP is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Ralpha) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naive T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFalpha) while down-regulating IL-10 and IFN-gamma suggesting a role in initiating allergic inflammation.

Product Properties

• Molecular Weight: Approximately 15.0 KDa, a single non-glycosylated polypeptide chain containing 132 amino acids.
• Source: Escherichia coli.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC L EQVSQLQG L WRRFNRPL LK QQ
• Formula: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Application Notes

• Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.