Beta defensin 3, also known as BD-3 and DEFB-3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 beta-defensins which are distinguished from alpha-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.
Molecular Weight:
Approximately 4.6 kDa, a single non-glycosylated polypeptide chain containing 41 amino acid residues.
Source:
Escherichia coli.
Purity:
>95% by SDS-PAGE and HPLC analyses.
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence:
KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K
Formula:
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Application Notes:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions
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