Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity . The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes . The 55 kDa IL-2 Ralphais specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rbeta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chaingammac/IL-2 Rgamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rbetaandgammac. It drives resting T cells to proliferate and induces IL-2 and IL-2 Ralphasynthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naive CD4+ T cells but not activated CD4+ memory lymphocytes . IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells .
Molecular Weight:
Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
Source:
Escherichia coli.
Purity:
>95% by SDS-PAGE and HPLC analyses.
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Application Notes:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.
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