KLH conjugated synthetic peptide derived from human RNF7/CKBBP1 (30-120/113aa)
Conjugation:
Unconjugated
Alternative Names:
CKBBP 1, CKBBP1, CKII beta binding protein 1, CKII beta-binding protein 1, Rbx 2, Rbx2, RBX2_HUMAN, Regulator Of cullins 2, RING box protein 2, Ring finger protein 7, RING-box protein 2, RNF 7, RNF7, ROC 2, ROC2, SAG, Sensitive to apoptosis gene, Sensitive to apoptosis gene protein, Zinc RING finger protein SAG.
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
RNF7
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF7) Polyclonal Antibody, Unconjugated (orb100014) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF7) Polyclonal Antibody, Unconjugated (orb100014) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RNF7 Polyclonal Antibody, Unconjugated (orb100014) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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