GDF10 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: GDF10 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB100085
• Supplier Catalog Number: orb100085
• Alternative Catalog Number: BYT-ORB100085-100, BYT-ORB100085-200, BYT-ORB100085-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human GDF10 (371-478/478aa)
• Conjugation: Unconjugated
• Alternative Names: BIP, BMP-3b, BMP3B, GDF10_HUMAN, GDF10, GDF-10, Bone morphogenetic protein 3B (BMP-3B), Bone-inducing protein (BIP), GDF10_MOUSE,

Product Description

GDF10 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 12/53 kDa
• UniProt: P55107
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: GDF10

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GDF10) Polyclonal Antibody, Unconjugated (orb100085) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Pancreas tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-GDF10 (orb100085) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12 kDa, Observed band size: 53 kDa.