KLH conjugated synthetic peptide derived from human ERN2 (465-550/926aa)
Conjugation:
Unconjugated
Alternative Names:
ER to nucleus signalling 2, ERN 2, hIRE2p, Inositol requiring 1 beta, Ire1 beta, IRE1 S. cerevisiae homolog of, IRE2, Serine/threonine protein kinase/endoribonuclease IRE2, ERN2_HUMAN, Endoplasmic reticulum-to-nucleus signaling 2, Inositol-requiring protein 2 (hIRE2p), Ire1-beta (IRE1b).,
ERN2 Rabbit Polyclonal Antibody
Clonality:
Polyclonal
Concentration:
1mg/ml
Molecular Weight:
98 kDa
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
ERN2
Application Dilute:
Flow-Cyt=2ug/Test
Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-ERN2 antibody (orb100216), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/test. Protocol, The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
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