0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
DNASE1L3
Application Dilute:
IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
Tissue/cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37?? for 20 min, Incubation: Anti-DNase gamma Polyclonal Antibody, Unconjugated (orb100610) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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