KLH conjugated synthetic peptide derived from human DBC2 (631-727/727aa)
Conjugation:
Unconjugated
Alternative Names:
DBC2, DEE64, EIEE64, p83, E130206H14Rik, mKIAA0717, RHBT2_HUMAN, RHOBTB2, Deleted in breast cancer 2 gene protein, KIAA0717, RHBT2_MOUSE, Deleted in breast cancer 2 gene protein homolog,
0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
RHOBTB2
Application Dilute:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Tissue/cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DBC2 Polyclonal Antibody, Unconjugated (orb100854) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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