Robo Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Robo Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB100869
• Supplier Catalog Number: orb100869
• Alternative Catalog Number: BYT-ORB100869-100, BYT-ORB100869-200, BYT-ORB100869-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Robo (51-150/1651aa)
• Conjugation: Unconjugated
• Alternative Names: CPHD8, DUTT1, NORS, NYS8, SAX3, Gm310, ROBO1_HUMAN, ROBO1, Deleted in U twenty twenty, H-Robo-1, ROBO1_MOUSE,

Product Description

Robo Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 181 kDa
• UniProt: Q9Y6N7
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ROBO1

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Robo Polyclonal Antibody, Unconjugated (orb100869) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.