ASCL1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: ASCL1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10143
• Supplier Catalog Number: orb10143
• Alternative Catalog Number: BYT-ORB10143-100,BYT-ORB10143-200,BYT-ORB10143-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human ASCL1 (151-236/236aa)
• Conjugation: Unconjugated
• Alternative Names: Achaete scute complex homolog 1, MASH1, MASH1/Achaete-scute homolog 1, Achaete scute complex homolog like 1, Achaete scute complex homologue 1, Achaete scute complex homologue like 1, Ascl 1, Ascl1, Ash 1, Ash1, Hash 1, Hash1, Mammalian achaete scute homolog 1, Mammalian achaete scute homologue 1, Mash 1, Achaete-scute homolog 1, ASCL1_HUMAN, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46.

Product Description

ASCL1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 26 kDa
• Pubmed: 25450527
• UniProt: P50553
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ASCL1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

Images

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (MASH1) polyclonal Antibody, Unconjugated (orb10143) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated (orb10143) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: mouse embryos tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated (orb10143) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated (orb10143) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated (orb10143) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-MASH1 antibody (orb10143), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse brain. Primary Antibody (green line): Rabbit Anti-MASH1 antibody (orb10143), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ASCL1 (orb10143) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD.