CDK4 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: CDK4 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10358
• Supplier Catalog Number: orb10358
• Alternative Catalog Number: BYT-ORB10358-100,BYT-ORB10358-200,BYT-ORB10358-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human CDK4 (241-303/303aa)
• Conjugation: Unconjugated
• Alternative Names: Cdk 4, CDK4 protein, Cell division kinase 4, Cell division protein kinase 4, CMM 3, CMM3, Crk3, Cyclin dependent kinase 4, Melanoma cutaneous malignant 3, MGC14458, p34 cdk4, PSK J3, CDK4_HUMAN.

Product Description

CDK4 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 34 kDa
• UniProt: P11802
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CDK4

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Images

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (orb10358) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (orb10358) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Rat) Lysate at 30 ug, Colon carcinoma (Human) lysate at 30 ug, Primary: Anti-CDK4 (orb10358) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-CDK4 (orb10358) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CDK4 (orb10358) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (CDK4) polyclonal Antibody, Unconjugated (orb10358) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (Red line): Rabbit Anti-CDK4 antibody (orb10358), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (green line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (orb10358) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.