ChAT Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: ChAT Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10380
• Supplier Catalog Number: orb10380
• Alternative Catalog Number: BYT-ORB10380-100,BYT-ORB10380-200,BYT-ORB10380-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human ChAT (101-200/748aa)
• Conjugation: Unconjugated
• Alternative Names: Choline O acetyltransferase, Choline O acetyltransferase, Acetyl CoA choline O acetyltransferase, Acetyl CoA:choline O acetyltransferase, ChAT, CHOACTase, Choline acetylase, choline acetyltransferase, CMS1A, CMS1A2, EC 2.3.1.6, OTTHUMP00000019583, OTTHUMP00000019584, CLAT_HUMAN.

Product Description

ChAT Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 82 kDa
• UniProt: P28329
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CHAT

Application Notes

• Application Dilute: WB=1:500-2000, Flow-Cyt=1ug/Test

Images

Blank control (Black line): Molt-4 (Black). Primary Antibody (green line): Rabbit Anti-ChAT antibody (orb10380), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated (orb10380) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ChAT) Polyclonal Antibody, unconjugated (orb10380) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated (orb10380) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human U251 cell lysates, Primary: Anti-ChAT (orb10380) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Ra

Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-ChAT antibody (orb10380), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-ChAT antibody (orb10380), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated (orb10380) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.