CREB-1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: CREB-1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10458
• Supplier Catalog Number: orb10458
• Alternative Catalog Number: BYT-ORB10458-50,BYT-ORB10458-100,BYT-ORB10458-200
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human CREB-1 (101-150/341aa)
• Conjugation: Unconjugated
• Alternative Names: Active transcription factor CREB, cAMP response element binding protein, cAMP responsive element binding protein 1, CREB1, CREB 1, CREB1, MGC9284, Transactivator protein, CREB, CREB1_HUMAN.

Product Description

CREB-1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 37 kDa
• UniProt: P16220
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CREB1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CREB-1 Polyclonal Antibody, Unconjugated (orb10458) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CREB-1) Polyclonal Antibody, Unconjugated (orb10458) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-CREB-1 (orb10458) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.

Sample: Hela Cell Lysate at 40 ug, Primary:Anti-CREB-1 (orb10458) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.