HSP27 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: HSP27 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10845
• Supplier Catalog Number: orb10845
• Alternative Catalog Number: BYT-ORB10845-100,BYT-ORB10845-200,BYT-ORB10845-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human HSP27 (101-205/205aa)
• Conjugation: Unconjugated
• Alternative Names: Heat shock 27kDa protein, 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, Heat Shock Protein27, Heat Shock Protein27, HMN2B, HS.76067, Hsp 25, Hsp 25, Hsp 27, Hsp 27, Hsp 28, Hsp 28, Hsp B1, Hsp B1, Hsp25, Hsp25, Hsp28, Hsp28, HspB1, HspB1, HSPB1_HUMAN, SRP27, Stress responsive protein 27, Stress-responsive protein 27.

Product Description

HSP27 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 27 kDa
• UniProt: P04792
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: HSPB1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2µg/Test

Images

Blank control (blue line): A431 cells (blue). Primary Antibody (green line): Rabbit Anti-HSP27 antibody (orb10845), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb10845) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb10845) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb10845) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb10845) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) lysate at 30 ug, Liver (Mouse) lysate at 30 ug, Primary: Anti-HSP-27 (orb10845) at 1:200, Secondary: AP conjugated Goat Anti-Rabbit IgG at 1:3000 dilution, NBT/BCIP staining, Predicted band size: 27kD, Observed band size: 27kD.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: Huvec (Human) Cell Lysate at 30 ug, Lane 5: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP27 (orb10845) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27-30 kD, Observed band size: 27 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HSP-27 Polyclonal Antibody, Unconjugated (orb10845) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.