Integrin beta 1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Integrin beta 1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB10944
• Supplier Catalog Number: orb10944
• Alternative Catalog Number: BYT-ORB10944-100,BYT-ORB10944-200,BYT-ORB10944-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin beta 1 (25-100/798aa)
• Conjugation: Unconjugated
• Alternative Names: Integrin beta-1, beta 1 subunit, CD 29, CD-29, CD29, CD29 antigen, Fibrinogen Receptor beta subunit, Fibronectin receptor subunit beta, Fibronectin receptor, beta subunit, FNRB, GPIIA, GP IIa, Integrin VLA 4 subunit beta, ITGB, ITGB1, ITGB 1, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, Glycoprotein IIa, Integrin VLA 4 subunit beta, integrin VLA-4 beta subunit, ITB1_HUMAN, very late activation protein, beta polypeptide, VLA BETA, VLA-4 subunit beta, VLA-BETA, VLAB, VLAbeta, Integrin beta1.

Product Description

Integrin beta 1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 130kD kDa
• Pubmed: 28806534
• UniProt: P05556
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ITGB1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Images

Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb10944), Dilution: 3 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (orb10944) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (orb10944) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (orb10944) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: U-87MG (Human) Cell Lysate at 30 ug, Lane 3: Ear (Rat) Lysate at 40 ug, Lane 4

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb10944), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HUVEC cells (blue). Primary Antibody: Rabbit Anti-CD29 antibody (orb10944), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10944, 1 µg/1x10 6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Integrin beta 1) Polyclonal Antibody, Unconjugated (orb10944) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.