This Recombinant Human Semaphorin-4D (SEMA4D), partial (Active) spans the amino acid sequence from region 22-734aa. Purity: Greater than 95% as determined by SDS-PAGE.
Biological Origin: Homo sapiens (Human). Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 µg/mL can bind biotinylated Pepinemab, the EC50 is 1.005-1.776 ng/mL. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 2 µg/ml can bind biotinylated Pepinemab, the EC50 is 1.005-1.776 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D at 5 µg/ml can bind human PLXNB1, the EC50 is 0.8179-1.357 µg/mL.
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