Phospho-P38 MAPK (Thr180 + Tyr182) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-P38 MAPK (Thr180 + Tyr182) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB11208
• Supplier Catalog Number: orb11208
• Alternative Catalog Number: BYT-ORB11208-100,BYT-ORB11208-200,BYT-ORB11208-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182 M(p-T)G(p-Y)VA
• Conjugation: Unconjugated
• Alternative Names: P38 MAPK(phospho-Thr180), phospho-MAPK14(Thr180/Tyr182), MAPK14(phospho Thr180/Tyr182), CSAID Binding Protein 1, CSAID binding protein, CSAID-binding protein, Csaids binding protein, CSBP 1, CSBP 2, CSBP, CSBP1, CSBP2, CSPB 1, CSPB1, Cytokine suppressive anti inflammatory drug binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, MAX-interacting protein 2, Mitogen Activated Protein Kinase 14, Mitogen activated protein kinase p38 alpha, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, MK14_HUMAN, Mxi 2, Mxi2, p38 ALPHA, p38, p38 MAP kinase, p38 MAPK, p38 mitogen activated protein kinase, p38ALPHA, p38alpha Exip, PRKM14, PRKM15, RK, SAPK 2A, SAPK2A, Stress Activated Protein Kinase 2A.

Product Description

Phospho-P38 MAPK (Thr180 + Tyr182) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 42 kDa
• Pubmed: 28295929
• UniProt: Q16539
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MAPK14

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Phospho-P38 MAPK (Thr180 + Tyr182)antibody (orb11208, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37C. Primary antibody (orb11208, 1 µg/1x10 6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Muscle (Mouse) Lysate at 40 ug, Muscle (Rat) Lysate at 40 ug, Primary: Anti-Phospho-P38 MAPK (Thr180 + Tyr182) (orb11208) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-P38 MAPK (Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (orb11208) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-P38 MAPK (Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (orb11208) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-P38 MAPK (Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (orb11208) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody (orb11208), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-P38 MAPK) Polyclonal Antibody, Unconjugated (orb11208) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-P38 MAPK (Thr180 + Tyr182) (orb11208) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.