Phospho-P53 (Ser392) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-P53 (Ser392) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB11212
• Supplier Catalog Number: orb11212
• Alternative Catalog Number: BYT-ORB11212-100,BYT-ORB11212-200,BYT-ORB11212-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser392 PD(p-S)D
• Conjugation: Unconjugated
• Alternative Names: P53(phospho S392), P53(phospho Ser392), p-P53(Ser392), p-P53(S392), P53_HUMAN, Cellular tumor antigen p53, TP53, Antigen NY-CO-13,Phosphoprotein p53, Tumor suppressor p53, tumor protein p53, BCC7, LFS1, BMFS5, TRP53, P 53, P-53, wt-p53, Widespread p53, Wtp53, Wt p53,

Product Description

Phospho-P53 (Ser392) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 43 kDa
• UniProt: P04637
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: TP53

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ICC/IF=1:50-200
• Application Notes: Modification: Phosphorylated

Images

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-P53 (Ser392) antibody (orb11212), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-P53 (Ser392)) Polyclonal Antibody, Unconjugated (orb11212) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-P53 (Ser392)) Polyclonal Antibody, Unconjugated (orb11212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-P53 (Ser392)) Polyclonal Antibody, Unconjugated (orb11212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-P53 (Ser392)) Polyclonal Antibody, Unconjugated (orb11212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-P53 (Ser392) (orb11212) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 53 kD.

Tissue/Cell: mouse embryonic lung, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-P53 (Ser392) Polyclonal Antibody, Unconjugated (orb11212) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-P53 (Ser392) Polyclonal Antibody, Unconjugated (orb11212) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.