SFRP1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: SFRP1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB11362
• Supplier Catalog Number: orb11362
• Alternative Catalog Number: BYT-ORB11362-100,BYT-ORB11362-200,BYT-ORB11362-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human SFRP1 (201-314/314aa)
• Conjugation: Unconjugated
• Alternative Names: Secreted frizzled related protein 1, Frizzled related protein 1, FRP 1, SARP 2, SARP-2, Secreted apoptosis related protein 2, Secreted Apoptosis-related Protein 2, Secreted frizzled related protein, Secreted Frizzled-related Protein 1, Secreted frizzled-related protein, SFRP 1, sFRP-1, SFRP1, SFRP1_HUMAN, SFRP1, FRP, FRP-1, FRP1, FrzA, SARP2.

Product Description

SFRP1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 33 kDa
• UniProt: Q8N474
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SFRP1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Images

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Liver (Mouse) lysate at 30 ug, Primary: Anti-SFRP1 (orb11362) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-SFRP1 Polyclonal Antibody, Unconjugated (orb11362) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (orb11362) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.