SYVN1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: SYVN1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB11440
• Supplier Catalog Number: orb11440
• Alternative Catalog Number: BYT-ORB11440-100, BYT-ORB11440-200, BYT-ORB11440-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human SYVN1 (531-617/617aa)
• Conjugation: Unconjugated
• Alternative Names: DER3, HRD1, 1200010C09Rik, D530017H19Rik, RGD1310488, SYVN1_HUMAN, SYVN1, RING-type E3 ubiquitin transferase synoviolin, Synovial apoptosis inhibitor 1, 2.3.2.27, KIAA1810, SYVN1_MOUSE,

Product Description

SYVN1 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 65 kDa
• UniProt: Q86TM6
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SYVN1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3µg/Test

Images

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

The figure annotation: The blue histogram is unstained cells. The Wathet Blue histogram is cells stained with secondary antibody (orb868805) alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody (orb570250) plus secondary antibody. The green histogram is

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (orb11440) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Liver (Mouse) Lysate at 40 ug, Primary: Anti-SYVN1 (orb11440) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65/70 kD.