Keratin 6 Recombinant Rabbit Monoclonal Antibody, Clone: [B12C5], Unconjugated

Product Overview

• Article Name: Keratin 6 Recombinant Rabbit Monoclonal Antibody, Clone: [B12C5], Unconjugated
• Biozol Catalog Number: BYT-ORB1151961
• Supplier Catalog Number: orb1151961
• Alternative Catalog Number: BYT-ORB1151961-100,BYT-ORB1151961-25,BYT-ORB1151961-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Keratin 6
• Conjugation: Unconjugated
• Alternative Names: Keratin, type II cytoskeletal 6C, KRT6C, Cytokeratin-6C, CK-6C, Cytokeratin-6E, CK-6E, Keratin K6h, Keratin-6C, K6C, Type-II keratin Kb12, KRT6E, K2C6C_HUMAN

Product Description

Keratin 6 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [B12C5]
• Molecular Weight: 60 kDa
• UniProt: P48668
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KRT6C

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

Images

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: A431, Rat skin, Mouse skin, Protein loading quantity: 20 µg, Exposure time: 15 s, Predicted MW: 60 kDa, Observed MW: 56 kDa.

Cell line: HaCat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151961.

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 6) Monoclonal Antibody, Unconjugated (orb1151961) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.