STMN1 Recombinant Rabbit Monoclonal Antibody, Clone: [S9H7], Unconjugated

Product Overview

• Article Name: STMN1 Recombinant Rabbit Monoclonal Antibody, Clone: [S9H7], Unconjugated
• Biozol Catalog Number: BYT-ORB1151977
• Supplier Catalog Number: orb1151977
• Alternative Catalog Number: BYT-ORB1151977-100,BYT-ORB1151977-25,BYT-ORB1151977-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human Stathmin 1 (100-149aa)
• Conjugation: Unconjugated
• Alternative Names: LAP18, C1orf215, Lag, LAP 18, LAP18, LAP-18, Leukemia associated phosphoprotein p18, Metablastin, Oncoprotein 18, OP 18, OP18, p18, p19, phosphoprotein 19, phosphoprotein p19, PP17, PP19, PR22, Pr22 protein, Prosolin, Protein Pr22, SMN, Stathmin, Stathmin1, Stathmin 1, Stathmin-1, STMN 1, STMN1_HUMAN.

Product Description

STMN1 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [S9H7]
• Molecular Weight: 17 kDa
• UniProt: P16949
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: STMN1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100

Images

Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151977.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (STMN1) Monoclonal Antibody, Unconjugated (orb1151977) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human SH-SY5Y cell lysates, Lane 7: Human U-2 OS cell lysates, Primary: Anti-STMN1 (orb1151977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 16 kDa.