The kit provides a method for detecting the O2- content in biological samples such as tissues, serum, and cells. The superoxide anion reacts with hydroxylamine hydrochloride to produce NO2-, which then reacts with Gris reagent.
Range:
0.0125-0.5 mg/mL
Sensitivity:
0.0125 mg/mL
Application Notes:
Application Notes: The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells . In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris reagent. The mechanism of Gris analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value
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