Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit

Product Overview

• Article Name: Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit
• Biozol Catalog Number: BYT-ORB1173218
• Supplier Catalog Number: orb1173218
• Alternative Catalog Number: BYT-ORB1173218-48, BYT-ORB1173218-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Alternative Names: Phosphopyruvate carboxylase, Phosphoenolpyruvic carboxylase, PHOSPHOENOLPYRUVATE CARBOXYLASE,Phosphoenolpyruvic acid cocarboxylase, Phospho(enol)pyruvate Carboxylase corn, Carboxylase, phosphopyruvate (phosphate)

Product Description

The kit provides a method for detecting PEPC activity in biological samples such as tissues, cell cultures, serum, and plasma. PEPC catalyzes the production of oxaloacetate, which is then converted to malate by malate dehydrogenase with the oxidation of NADH.

Application Notes

• Application Notes: Application Notes: The kit provides a simple method for detecting Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial. In the assay, Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The rate of NADH decrease at 340 nm can reflect Phosphoenolpyruvate carboxylase (PEPC) activity