This kit detects AST/GOT activity by catalyzing the transamination of alpha-ketoglutarate and L-aspartic acid to produce pyruvate. The rate of absorbance increase at 505 nm is used to calculate AST/GOT activity.
Application Notes:
Application Notes: The kit provides a simple method for detecting AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of alpha-ketoglutarate and L-aspartic acid at 37C and pH 7.4 to produce glutamic acid and pyruvate, Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST/GOT activity
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