Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit
Biozol Catalog Number:
BYT-ORB1173230
Supplier Catalog Number:
orb1173230
Alternative Catalog Number:
BYT-ORB1173230-48, BYT-ORB1173230-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol, phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm. The absorbance increase rate reflect AKP activity.
Application Notes:
Application Notes: In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol, phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm, the absorbance increase rate reflect AKP activity
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