GR can catalyze the reduction of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP+, NADPH has a characteristic absorption peak at 340 nm, while NADP+ has no absorption peak at this wavelength. NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GR activity.
Application Notes:
Application Notes: GR can catalyze the reduction of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP +, NADPH has a characteristic absorption peak at 340 nm, while NADP + has no absorption peak at this wavelength, NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GR activity
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