Coenzyme 1 NAD(H) Assay Kit provides a tool for detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NAD+ /NADH concentration in the sample.
Range:
0.78 µM-50 µM
Application Notes:
Application Notes: Coenzyme 1 NAD(H) Assay Kitprovides a convenient tool for sensitive detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NAD+ /NADH concentration in the sample
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