CD68 Recombinant Rabbit Monoclonal Antibody, Clone: [3G6], Unconjugated

Product Overview

• Article Name: CD68 Recombinant Rabbit Monoclonal Antibody, Clone: [3G6], Unconjugated
• Biozol Catalog Number: BYT-ORB1183943
• Supplier Catalog Number: orb1183943
• Alternative Catalog Number: BYT-ORB1183943-25,BYT-ORB1183943-50,BYT-ORB1183943-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human CD68
• Conjugation: Unconjugated
• Alternative Names: GP110, LAMP4, SCARD1, CD68_HUMAN, CD68, CD68_MOUSE,

Product Description

CD68 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [3G6]
• Molecular Weight: 37 kDa
• UniProt: P34810
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CD68

Application Notes

• Application Dilute: IHC-P=1:500-1000, IHC-F=1:500-1000, IF=1:500-1000, Flow-Cyt=1ug/Test

Images

4% Paraformaldehyde-fixed THP-1 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (CD68) monoclonal Antibody, unconjugated (orb1183943) 1:100, 90 min at 37C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37C for 90 min, DAPI (blue) was used to stain the cell nuclei.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD68 antibody (orb1183943), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD68) Monoclonal Antibody, Unconjugated (orb1183943) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of human tonsil using CD68 Monoclonal Antibody at 1:200 dilution.