BI-1/TMBIM6/BI Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: BI-1/TMBIM6/BI Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1184701
• Supplier Catalog Number: orb1184701
• Alternative Catalog Number: BYT-ORB1184701-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human BI-1/TMBIM6, identical to the related mouse and rat sequences.
• Conjugation: Unconjugated
• Alternative Names: Bax inhibitor 1, BAXI1, BI 1, BI1, TEGT, TMBIM6

Product Description

Anti-BI-1/TMBIM6 Antibody. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Properties

• Clonality: Polyclonal
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molecular Weight: 35 kDa
• UniProt: P55061
• Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Form: Lyophilized
• Target: Bax inhibitor 1

Application Notes

• Application Dilute: Western blot, 0.25-0.5 µg/ml/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml/ml, Human Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunofluorescence, 5 µg/ml, Human

Images

IF analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-BI-1/TMBIM6 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each l

IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody. BI-1/TMBIM6 was detected in a pa