NKCC1/SLC12A2 Mouse Monoclonal Antibody, Clone: [6G7D2], Unconjugated

Product Overview

• Article Name: NKCC1/SLC12A2 Mouse Monoclonal Antibody, Clone: [6G7D2], Unconjugated
• Biozol Catalog Number: BYT-ORB1184753
• Supplier Catalog Number: orb1184753
• Alternative Catalog Number: BYT-ORB1184753-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: E.coli-derived human NKCC1/SLC12A2 recombinant protein (Position: K889-K943).
• Conjugation: Unconjugated
• Alternative Names: Basolateral Na K Cl symporter, BSC, BSC2, NKCC1, SLC12A2

Product Description

Anti-NKCC1/SLC12A2 Antibody (monoclonal, 6G7D2). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Properties

• Clonality: Monoclonal
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Clone Designation: [6G7D2]
• Molecular Weight: 200 kDa
• UniProt: P55011
• Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Form: Lyophilized
• Target: Solute carrier family 12 member 2

Application Notes

• Application Dilute: Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunofluorescence, 5 µg/ml, Human, Mouse, Rat

Images

IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight550 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight550 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight550 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-NKCC1/SLC12A2 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay K

IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detec