AREB6/ZEB1 Mouse Monoclonal Antibody, Clone: [8B12D7], Unconjugated

Product Overview

• Article Name: AREB6/ZEB1 Mouse Monoclonal Antibody, Clone: [8B12D7], Unconjugated
• Biozol Catalog Number: BYT-ORB1184754
• Supplier Catalog Number: orb1184754
• Alternative Catalog Number: BYT-ORB1184754-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human AREB6/ZEB1.
• Conjugation: Unconjugated
• Alternative Names: AREB6, BZP, DELTA EF1, delta EF1, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A

Product Description

Anti-AREB6/ZEB1 Antibody (monoclonal, 8B12D7). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Properties

• Clonality: Monoclonal
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Clone Designation: [8B12D7]
• Molecular Weight: 200 kDa
• UniProt: P37275
• Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Form: Lyophilized
• Target: Zinc finger E-box-binding homeobox 1

Application Notes

• Application Dilute: Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunofluorescence, 5 µg/ml, Human

Images

IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-AREB6/ZEB1 Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight550 Conjugated Avidin. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-AREB6/ZEB1 Antibody overnight at 4C. DyLight550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue secti