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Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with orb1240153, orb1238174, orb1240159, orb1240157, orb1240154, orb1240156, and orb1240178 antibodies at decreasing concentrations. |
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Western blot analysis of TIM-3 in over expressing HEK293 cells using orb1240153, orb1240157, and orb1240154 antibodies at (A) 0.25 µg/ml, (B) 0.5 µg/ml, and (C) 1 µg/ml. |
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Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) control mouse IgG antibody at 1 µg/ml. |
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Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 10 µg/ml. |
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Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 20 µg/ml. |
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Immunohistochemistry of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 5 µg/ml. |
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Flow cytometry analysis of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) mouse IgG at 0.1 µg/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells. |