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Titration curve analysis of PD-L1 mAbs to detect recombinant PD-L1 in ELISA with orb1239789, orb1239794, orb1239852, orb1239770, orb1239795, orb1239796, and orb1239818 antibodies at decreasing concentrations. |
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Western blot analysis of PD-L1 in over expressing HEK293 cells using orb1239789, orb1239794, orb1239770, orb1239795, orb1239796, and orb1239818 antibodies at 0.25 µg/ml and 0.5 µg/ml. |
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Immunocytochemistry of PD-L1 in over expressing HEK293 cells using (A) orb1239789, (B) orb1239794, (C) orb1239852, (D) orb1239770, (E) orb1239795, (F) orb1239796, (G) orb1239818, and (H) control mouse IgG antibody at 1 µg/ml. |
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Immunofluorescence of PD-L1 in over expressing HEK293 cells using (A) orb1239789, (B) orb1239794, (C) orb1239852, (D) orb1239770, (E) orb1239795, (F) orb1239796, and (G) orb1239818 antibody at 2 µg/ml. |
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Immunofluorescence of PD-L1 in human stomach carcinoma tissue using (A) orb1239789, (B) orb1239794, (C) orb1239852, (D) orb1239770, (E) orb1239795, (F) orb1239796, and (G) orb1239818 antibody at 2 µg/ml. |
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Immunohistochemistry of PD-L1 in human stomach carcinoma tissue using (A) orb1239789, (B) orb1239794, (C) orb1239852, (D) orb1239770, (E) orb1239795, (F) orb1239796, (G) orb1239818, and (H) control mouse IgG antibody at 10 µg/ml. |
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Flow cytometry analysis of PD-L1 in over expressing HEK293 cells using (A) orb1239789, (B) orb1239794, (C) orb1239852, and (D) mouse IgG at 0.1 µg/ml. Blue: untransfected HEK293 cells. Yellow: PD-L1 over expressing HEK293 cells. |