SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein Preis auf Anfrage

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein Preis auf Anfrage
• Biozol Catalog Number: BYT-ORB1228206
• Supplier Catalog Number: orb1228206
• Alternative Catalog Number: BYT-ORB1228206-0.05
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: ELISA, SDS-PAGE, WB
• Alternative Names: Delta variant spike RBD, S glycoprotein RBD, S protein RBD, Spike glycoprotein Receptor-binding domain, Spike protein RBD, covid-19, sars-cov-2

Product Description

SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein

Product Properties

• Molecular Weight: 120 kD
• NCBI: 43416
• UniProt: P0DTC1
• Buffer: PBS (pH 7.4).
• Purity: > 90% as determined by Bis-Tris PAGE
• Form: Liquid
• Sequence: Val 16 - Arg 685 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R)-His-Avi
• Target: S

Application Notes

• Application Notes: Application Notes: This recombinant protein can be used for WB and ELISA. For research use only

Images

ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein. Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 µg/mL, incubated at 4 C overnight. Detection Antibodies: ACE2 recombinant protein, orb1231604, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-human HRP at 1:10, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein. Loading: 1 µg SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, orb1228206. Observed: 120kD

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 µg/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, orb1239050, and spike 156-157EF antibody, orb1239051, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant Spike S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibody (orb1239050), while the corresponding WT antibody (orb1239051) detected spike S1 protein of WT, but not delta variant.

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 µg/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant Spike S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibodies.

WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, orb1239050, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibody (orb1239050).

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 µg/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 antibody, orb1239995, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. Antibody orb1239995 does not distinguish between WT and Delta.