Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human CD27, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human CD27, His Tag at 2 ug/ml (100 uL/well) can bind Biotinylated Human CD27 Ligand, Avitag, Fc Tag with a linear range of 2-31 ng/mL.
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