Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 10.9 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Mouse C5a, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Mouse C5a, His Tag at 2 ug/ml (100 uL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL.
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