Application Notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 73.9 kDa (beta chain) and 114.4 kDa (alpha
Human Complement C5, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Complement C5, His Tag at 2 ug/ml (100 uL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.2-3 ng/mL.
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