Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human IL-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human IL-5, His Tag at 5 ug/ml (100 uL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 0.02-0.313 ug/ml.
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