Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 96.6 kDa. The protein migrates as 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human LRP-5, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human Dkk-1, Fc, Avitag at 5 ug/ml (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.5-16 ng/mL.
Immobilized Human Dkk-1, His Tag at 5 ug/ml (100 uL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.01-0.313 ug/ml.
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