Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human IL-13, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse IL-13RA1, Fc Tag at 2 ug/ml (100 uL/well) can bind Human IL-13, His Tag with a linear range of 5-39 ng/mL.
Immobilized Human IL-13, His Tag at 5 ug/ml (100 uL/well) can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.3-5 ng/mL.
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