Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. the protein migrates as 16-17 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE)
Human GDF-15, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human GDF-15, His Tag at 1 ug/ml (100 uL/well) can bind Human GFR alpha-like Fc Chimera Protein with a linear range of 0.2-8 ng/mL.
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